Scientist / Sr Scientist / Staff Scientist -… at Twist… · South…

Summary: Twist Bioscience is searching for a curious, thoughtful, and driven RnD scientist to enhance their antibody development platform. The role involves using scientific expertise in various antibody modalities to create and improve platform technologies for antibody discovery and development. Responsibilities: - Develop antibody production, purification and analytical workflows at various scales and throughput, across various formats (single-chain, IgG, multi-specific, Fc-Fusion, etc) - Optimize all aspects of the end-to-end antibody development workflow including but not limited to: construct design, protein expression (transient and stable), purification, and analytics - Independently design and execute experiments, and analyze experimental data - As appropriate, build and lead a team to achieve technical goals - Move projects forward efficiently / manage time and resources effectively / prioritize tasks in accordance with the company’s goals - Document and communicate results clearly and succinctly - Be helpful / collaborative / proactive - Stay up to date with new developments, companies, and technologies - Be the ultimate subject matter expert, and understand all aspects of your work as profoundly as possible, including details that are adjacent to your projects Required Qualifications: - Deep hands-on experience designing, expressing, and optimizing monoclonal antibodies, with demonstrated understanding of Fc structure–function relationships (effector function, stability, manufacturability) - Proven experience with bispecific antibody architectures, including but not limited to: Knob-in-hole, CrossMab, common light chain, or similar - Chain pairing control, mispairing mitigation, and associated analytics - Extensive experience generating and optimizing stable mammalian cell lines (CHO preferred): Vector design, promoter/selection strategies, Clone screening, productivity optimization, and stability assessment - Strong understanding of transient vs stable expression tradeoffs and when to deploy each during platform development - Experience improving expression yields and product quality through sequence, vector, and host engineering - Demonstrated ability to build, improve, and standardize antibody generation workflows - Experience developing high-throughput or semi-automated workflows for expression, purification, and screening - Strong understanding of platform robustness, reproducibility, and cost drivers - Hands-on experience with antibody characterization methods, including but not limited to: SEC, CE-SDS, Binding kinetics (BLI or SPR), Basic biophysical profiling (DSF, DLS), Mass spectrometry - Strong mechanistic understanding of antibody biology, structure, and expression bottlenecks - Experience designing platforms that support multiple antibody modalities (mAbs, bispecifics, Fc-engineered variants) - A track record of successful and creative method development - Advanced degree in Cell Biology, Biochemistry, Chemical Engineering, or a closely-related field - Scientist Requires a PhD and 0-2 years of previous professional experience; or a master's degree and 3-5 years of work experience - Sr Scientist Requires a Ph.D. and 3-5 years of professional experience (postdoc may count); or a master's degree and 7+ years of work experience - Staff Scientist Requires a Ph.D. and 6-10 years of professional experience (postdoc may count); or a master's degree and 10+ years of work experience Preferred Qualifications: - Familiarity with Python or equivalent programming languages is a plus - Experience with liquid-handling systems and automation is a plus Required Skills: Antibody development, Monoclonal antibody design, Fc structure-function knowledge, Bispecific antibody architectures, Knob-in-hole, CrossMab, Common light chain, Chain pairing control, Mispairing mitigation, Stable mammalian cell line generation, CHO cell line experience, Vector design, Promoter and selection strategies, Clone screening, Productivity optimization, Stability assessment, Transient and stable protein expression, Sequence engineering, Vector engineering, Host engineering, Antibody production workflows, Antibody purification workflows, Antibody analytical workflows, High-throughput workflow development, Semi-automated workflow development, Antibody characterization methods, Size exclusion chromatography (SEC), Capillary electrophoresis SDS (CE-SDS), Binding kinetics (BLI, SPR), Biophysical profiling (DSF, DLS), Mass spectrometry, Python programming, Liquid-handling automation, Collaboration, Proactivity Benefits: Bonus, Equity, Generous benefits package