Research Assistant I NEUROLOGY kl at Mass General Brigham in Boston-MA

Summary: Mass General Brigham is a not-for-profit organization that supports patient care, research, teaching, and community service. The Research Assistant will work under the supervision of a senior researcher, assisting in a range of molecular biology tasks and coordinating research activities, including data collection and management. Responsibilities: - Maintenance, passaging, cataloguing, and culturing of iPSC cells - Differentiation iPSCs to neurons and glia and aging of these cultures - Maintenance of Attune flow cytometry and ordering the related reagents - Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope - Maintenance of HEK293 cells, generation of stable cell lines - Helping with molecular cloning; Gibson assembly, gateway cloning, mini/maxi preps - Analysis of data from western blot/ microscopy/ flow cytometry - The candidate will have excellent lab note-keeping habits, mostly digital(LabArchieves) and will generate Standard Operating Procedures (SOPs) for the lab - The candidate will have excellent organizational skills, labeling of experiments, file management - The candidate will report daily to the immediate supervisor and weekly to the Principal investigator - The candidate will interact with junior and senior members of the lab to troubleshoot experiments - The candidate will share lab duties with other technicians - Responsible for data collection, management, manipulation, analysis, and reporting - Study cohort management - Outreach communication with subjects, conducting mailings, follow-up on mailings, and obtaining necessary documents - Provides assistance on work related to epidemiological studies and other population-based research - Performs other duties as assigned - Complies with all policies and standards Required Qualifications: - Good cell culture experience with human cells (HEK293 or any other immortalized line) - Excellent oral and written communication skills - Computer literacy, including database tools - Ability to follow directions and exhibit professionalism Required Skills: Cell Culture, HEK293 Cells, Induced Pluripotent Stem Cells (iPSC), Neuronal Differentiation, Flow Cytometry, Longitudinal Imaging, Incucyte, Nikon Biostation Microscope, Molecular Cloning, Gibson Assembly, Gateway Cloning, Western Blot, Microscopy, Data Management, Standard Operating Procedures (SOPs), LabArchives Benefits: Comprehensive benefits, Career advancement opportunities, Differentials, Premiums and bonuses, Recognition programs designed to celebrate your contributions and support your professional growth