Research Assistant I, Genetics at Mass General Brigham in Boston-MA

Summary: Mass General Brigham is a not-for-profit organization that supports patient care, research, teaching, and community service. They are seeking a Research Assistant I to assist in research focusing on the human immune system's response to cancers and how cancers evolve, utilizing techniques in molecular biology and cellular analysis. Responsibilities: - Perform standard molecular biology techniques (cloning, site-directed mutagenesis, PCR, and next-generation sequencing) - Perform tissue culture work doing plasmid transfection and retroviral infection - Characterize cellular function using biochemistry and cell biology techniques (Western blotting, immunofluorescence, chromatography, flow cytometry, CRISPR, and reporter gene assays) - Perform and analyze immunologic assays in cell culture setting to assess B- and T-cell specificity and activity, as well as molecular panning experiments to assess antibody specificity and function - Assist in CRISPR functional genomic screen to identify genes required for the selective survival of tumor cells - Organize and summarize acquired data, using scientific and statistical techniques Required Qualifications: - Perform standard molecular biology techniques (cloning, site-directed mutagenesis, PCR, and next-generation sequencing) - Perform tissue culture work doing plasmid transfection and retroviral infection - Characterize cellular function using biochemistry and cell biology techniques (Western blotting, immunofluorescence, chromatography, flow cytometry, CRISPR, and reporter gene assays) - Perform and analyze immunologic assays in cell culture setting to assess B- and T-cell specificity and activity, as well as molecular panning experiments to assess antibody specificity and function - Assist in CRISPR functional genomic screen to identify genes required for the selective survival of tumor cells - Organize and summarize acquired data, using scientific and statistical techniques - Bachelor's degree in biology, biochemistry, chemistry, biotechnology or related science field - Excellent communication skills, both oral and written - Attention to detail - Familiarity with molecular and computational biology - Knowledge of sterile tissue culture technique - Excellent organizational and analytical skills Preferred Qualifications: - 1-3 years of experience in molecular laboratory Required Skills: Molecular biology techniques, Cloning, Site-directed mutagenesis, PCR, Next-generation sequencing, Tissue culture, Plasmid transfection, Retroviral infection, Western blotting, Immunofluorescence, Chromatography, Flow cytometry, CRISPR, Reporter gene assays, Immunologic assays, Molecular panning, Functional genomic screening, Data analysis, Statistical techniques, Sterile tissue culture technique, Computational biology Benefits: Comprehensive benefits, Career advancement opportunities, Differentials, Premiums and bonuses